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si10 brevican  (Addgene inc)


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    Structured Review

    Addgene inc si10 brevican
    Si10 Brevican, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si10+brevican/SI10-Brevican+(Plasmid+%2346300)/pm41430471-388-4-5
    Average 93 stars, based on 1 article reviews
    si10 brevican - by Bioz Stars, 2026-09
    93/100 stars

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    Transfection:

    Article Title: Extracellular matrix proteolysis maintains synapse plasticity during brain development
    Article Snippet: The following primary antibodies were used: anti-GFP chicken polyclonal antibody (Aves Labs GFP-1020, 1:1000), anti-brevican mouse monoclonal antibody (1:100) , Living Colors DsRed polyclonal antibody (Clontech 632496, 1:1000), anti-SV2 mouse monoclonal antibody (DSHB, 1:500), anti-4C4 mouse monoclonal antibody (Gift from Hitchcock lab, 1:200), anti-HA rabbit monoclonal antibody (Cell Signaling Technology 3724T, 1:500). .. To obtain anti-brevican antibody, SI10-brevican (Addgene, #46300) was transfected into HEK293 cells by Lipofectamine 3000 Transfection reagent (Thermo Scientific) and collected culture media at 48 or 72 hours after transfection. .. The culture media was concentrated by Amicon Ultra centrifuge filter (Millipore) to 1/10 volume and used for staining at 1:100.

    Article Title: Extracellular matrix proteolysis maintains synapse plasticity during brain development.
    Article Snippet: Antibodies were: chicken anti-GFP (Aves Labs, cat. no. GFP-1020, 1:1,000), mouse anti-brevican (1:100)85, Living Colors DsRed (Clontech, cat. no. 632496, 1:1,000), mouse anti-SV2 (DSHB, 1:500), mouse anti-4C4 (gift from Hitchcock lab, 1:200), rabbit anti-HA (Cell Signaling Nature Neuroscience Article https://doi.org/10.1038/s41593-025-02153-4 Technology, cat. no. 3724T, 1:500), rabbit anti-synapsin (Synaptic Systems, cat. no. 106 002, 1:1,000), rat anti-mCherry (16D7) (Thermo Fisher Scientific, cat. no. M11217, 1:1,000), rabbit anti-MBP (1:500, gift from Appel lab86). .. To obtain anti-brevican antibody, SI10-brevican (Addgene, cat. no. 46300) was transfected into HEK293T cells by Lipofectamine 3000 Transfection reagent (Thermo Fisher Scientific) and collected from culture media at 48–72 h and concentrated to 1/10 volume (Amicon Ultra centrifuge filter, Millipore). .. The Wisteria floribunda lectin conjugated with biotin (Sigma-Aldrich, cat. no. L1516, 1:1,000) was mixed with the primary antibody solution.

    Article Title: Extracellular matrix proteolysis maintains synapse plasticity during brain development
    Article Snippet: Antibodies were: chicken anti-GFP (Aves Labs, cat. no. GFP-1020, 1:1,000), mouse anti-brevican (1:100) , Living Colors DsRed (Clontech, cat. no. 632496, 1:1,000), mouse anti-SV2 (DSHB, 1:500), mouse anti-4C4 (gift from Hitchcock lab, 1:200), rabbit anti-HA (Cell Signaling Technology, cat. no. 3724T, 1:500), rabbit anti-synapsin (Synaptic Systems, cat. no. 106 002, 1:1,000), rat anti-mCherry (16D7) (Thermo Fisher Scientific, cat. no. M11217 , 1:1,000), rabbit anti-MBP (1:500, gift from Appel lab ). .. To obtain anti-brevican antibody, SI10-brevican (Addgene, cat. no. 46300) was transfected into HEK293T cells by Lipofectamine 3000 Transfection reagent (Thermo Fisher Scientific) and collected from culture media at 48–72 h and concentrated to 1/10 volume (Amicon Ultra centrifuge filter, Millipore). .. The Wisteria floribunda lectin conjugated with biotin (Sigma-Aldrich, cat. no. L1516, 1:1,000) was mixed with the primary antibody solution.



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    si10 brevican  (Addgene inc)
    93
    Addgene inc si10 brevican
    Si10 Brevican, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/si10+brevican/SI10-Brevican+(Plasmid+%2346300)/pm41430471-388-4-5
    Average 93 stars, based on 1 article reviews
    si10 brevican - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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